| 產品名稱 |
pIP82 |
| 商品貨號 |
B238837 |
| Designations |
pIP82 |
| GenBank Number |
X64810
|
| Species |
Homo sapiens, human |
| Depositors |
WJ Van De Ven |
| Applications |
produces protein proprotein convertase subtilisin/kexin type 1 neuroendocrine convertase 1 (pro-protein-convertase PC1) |
| Vector |
Construct size (kb): 6.099999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(+)
Intact vector size: 3.199
Type of vector: phagemid
Vector end: XbaI
Vector end: XbaI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII-SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PCSK1
Other gene symbols: NEC1
Genomic copy number: unique
Gene name: proprotein convertase subtilisin/kexin type 1
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q15-q21
Tissue: lung
Type of DNA: cDNA
Insert end: XbaI
Insert end: XbaI
Insert size (kb): 2.9
Cross references: DNA Seq. Acc.: X64810 Insert lengths(kb): 2.900000095367432 Tissue: lung Gene product: proprotein convertase subtilisin/kexin type 1(neuroendocrine convertase 1 (pro-protein-convertase PC1)) [NEC1] Target Gene: proprotein convertase subtilisin/kexin type 1 |
| Insert Size (kb) |
2.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--3.2, 2.9; BamHI--4.1, 2.0; EcoRI--3.5, 1.9, 0.65; PstI--3.3, 2.8; SacI--3.6, 2.6. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.12; EcoRI--1.82; BamHI--1.97. The 3' XbaI site is not cleaved by XbaI. Sequence analysis indicates that the site is present. Corresponds to nt 87-3039 of the sequence record. Contains the complete coding sequence (nt 190-2451 of the sequence record). |
| References |
Creemers JW, et al. Expression in human lung tumor cells of the proprotein processing enzyme PC1/PC3. Cloning and primary sequence of a 5 kb cDNA. FEBS Lett. 300: 82-88, 1992. PubMed: 1547893
Wim J Van De Ven, personal communication
|
