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編號:B163759

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產品名稱	4B2
商品貨號	B163759
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody reacts with all types of human leukocytes, but not with non-leukocytes. The antibody binds to protein A.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with human T cells. Spleen cells were fused with P3U1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Cellular Products	immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Tumorigenic	Yes, in irradiated pristane primed (BALB/c X DBA/2)F1 mice.
Comments	The antibody reacts with all types of human leukocytes, but not with non-leukocytes. The antibody binds to protein A. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵viable cells/mL. Maintain cultures at a cell concentration between 2 x 10⁵ and 1 x 10⁶cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC^® Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
Isotype	IgG2a
Name of Depositor	RM Steinman
Deposited As	mouse (B cell); mouse (myeloma)
References	. Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ. Press; 1987. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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